The oxidation of lactic acid by Mycobacterium phlei

Chromic Acid Oxidation of Lactic Acid Catalysed by Manganese(II)

B ak o re and N a r a in 1 studied the kinetics of the oxidation of a-hydroxy acids by chromium(VI) and found that addition of Mn(II) decreases the rate of oxidation to one half of that in absence of Mn(II), when the hydrogen ion concentration is in the range 0.1 to 0.4 M. They attributed this to the disproportionation of Cr(IV) by Mn(II) ions. K em p and W a t e r s 2 observed Mn(II) catalysis...

Genetic transfer in Mycobacterium phlei.

Isolation from Mycobacterium Phlei

Mycobactin is the name suggested for a growth factor, present in acid-fast bacteria, which is essential for the growth of Mycobacterium johnei. A preliminary account ofthe work reported here has already been published (Francis, Madinaveitia, Macturk & Snow, 1949). Myco. johnei is an organism first observed by Johne & Frothingham (1895) in cases of chronic enteritis of cattle. It could not be cu...

Mechanism of action and crystallization of lactic oxidative decarboxylase from Mycobacterium phlei.

Earlier phases of an investigation concerning the lactic oxidative decarboxylase’ from Mycobacterium phlei (1, 2) extended the initial observations of Edson (3) and Edson and Cousins (4). A similar type of enzyme, obtained from Mycobacterium avium, has been described by Yamamura et al. (5, 6). The flavoprotein character of the enzyme, as well as the absence of associated coenzymes, has been est...

Citrate formation in Mycobacterium phlei.

Although it is well known that citrate stimulates the growth of acid fast bacteria, the metabolic role of citrate is still obscure in this bacterial group. Washed suspensions of mycobacteria either fail to oxidize citrate or oxidize this substance slowly (Edson, 1951). On the other hand, clarified extracts of mycobacteria oxidize citrate to a-ketoglutarate (Geronimus, Gray, and Birkeland, 1949;...